ZEISS LSM 800 with Airyscan

ZEISS LSM 800 with Airyscan

ZEISS Laser Confocal Scanning Microscope is a flexible and compact microscope with four diode lasers (405, 488, 561 and 640 nm), complete with three highly sensitive GaAsP detection and fast linear scanning. Add Airyscan, the revolutionary detection concept from ZEISS, and gain 1.7 times higher resolution in all three dimensions – resulting in a 5 times smaller confocal volume. Push sensitivity beyond the limits of all conventional confocals.

  • Perform fast linear scanning
  • Profit from high productivity and throughput
  • Benefit from great flexibility in live cell imaging
  • Get uncompromised image quality
  • Perform precise quantitative measurements

Standard Operating Procedure

Microscope

Detection

  • Inverted stand: Axio Observer.Z1
  • Upright stands: Axio Imager.M2, Axio Imager.Z2, Axio Examiner.Z1*
  • Camera port
  • Manual or motorized stages
  • Incubation solutions
  • Fast Z piezo inserts (for inverted stands)
  • Defnite Focus.2
  • 2 channel Gallium Arsenide Phosphid (GaAsP) PMT or 2 channel multialkali (MA) PMT
  • 1 additional GaAsP PMT, MA PMT or Airyscan detector for 40× or 63× objectives
  • Electronically switchable illumination and detection module (ESID) or transmitted light detector (T-PMT) with halogen lamp (HAL)

Objectives

Software

  • C-APOCHROMAT
  • Plan-APOCHROMAT
  • LD Plan-APOCHROMAT
  • EC Plan-NEOFLUAR
ZEN (blue edition), recommended modules: Tiles & Positions, Experiment Designer, 3Dxl Viewer – powered by arivis®

Illumination

Diode lasers: 405, 488, 561 and 640 nm

Major Equipment